THIAMINE DIPHOSPHATASE: A HISTOCHEMICAL STUDY
نویسندگان
چکیده
منابع مشابه
Histochemical study on thiamine.
The authors have applied the thiochrome method for determining thiamine to histochemical investigations and could observe the localization of thiamine in cells and tissues (1-6). Independently of our study, v. Muralt (7) had already reported a investigation on histological pictures of thiamine in peri pheral nerves by the thiochrome method, which was however unavailable to the authors at that t...
متن کاملAllosteric Properties of Nucleoside Diphosphatase and Its Identity with Thiamine Pyrophosphatase*
A procedure has been described for the purification of nucleoside diphosphatase from bovine liver microsomes. The purified enzyme is shown, by analysis in the ultracentrifuge and by polyacrylamide disc gel electrophoresis, to be nearly homogeneous. The enzyme has an s20,u, of 4.9 S. Thiamine pyrophosphatase was purified together with nucleoside diphosphatase. Evidence has been obtained which in...
متن کاملEnhanced Activities of Blood Thiamine Diphosphatase and Monophosphatase in Alzheimer's Disease
BACKGROUND Thiamine metabolites and activities of thiamine-dependent enzymes are impaired in Alzheimer's disease (AD). OBJECTIVE To clarify the mechanism for the reduction of thiamine diphosphate (TDP), an active form of thiamine and critical coenzyme of glucose metabolism, in AD. METHODS Forty-five AD patients clinically diagnosed and 38 age- and gender-matched control subjects without dem...
متن کاملAllosteric properties of nucleoside diphosphatase and its identity with thiamine pyrophosphatase.
A procedure has been described for the purification of nucleoside diphosphatase from bovine liver microsomes. The purified enzyme is shown, by analysis in the ultracentrifuge and by polyacrylamide disc gel electrophoresis, to be nearly homogeneous. The enzyme has an s20,u, of 4.9 S. Thiamine pyrophosphatase was purified together with nucleoside diphosphatase. Evidence has been obtained which in...
متن کاملA Histochemical and Cytochemical Study
Localization of 5’-nucleotidase in the frog retina was investigated using histochemical and cytochemical techniques. Light-microscopic observations revealed the presence of this enzyme in the inner retinal layers (the nerve fiber layer, ganglion cell layer, and inner plexiform layer). Ultrastructural investigations revealed that the enzyme activity is associated with the plasma membranes of the...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1962
ISSN: 0022-1554,1551-5044
DOI: 10.1177/10.5.580